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產(chǎn)品詳情

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產(chǎn)品名稱：SMAD4293CellLysate
產(chǎn)品型號：
產(chǎn)品廠商：Abbiotec
產(chǎn)品文檔：

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簡單介紹：

SMAD4293CellLysate

詳情介紹：

Product details
Product details
Characteristics	Antigen standard for SMAD family member 4 (SMAD4) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Components	This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer.

Target details
Target details
Alternative Name	SMAD4
Background	Synonyms: MAD, mothers against decapentaplegic homolog 4, SMAD, mothers against DPP homolog 4, mothers against decapentaplegic, Drosophila, homolog of, 4, deleted in pancreatic carcinoma locus 4, SMAD 4, hSMAD4, MAD homolog 4, deletion target in pancreatic carcin, SMAD4, DPC4, JIP, MADH4
NCBI Accession	NP_005350
Research Area	Signaling, Transcription Factors

Application Details
Application Details
Application Notes	WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 μg lysate per lane.
Restrictions	For Research Use only

Handling
Handling
Format	Liquid
Buffer	Each lysate vial contains 0.1 mg lysate in 0.1 mL (1 mg/mL) of RIPA Buffer (50 mM Tris-HCl pH 7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1 % NP40). The loading buffer vial contains 0.5 mL 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10 % glycerol, 4 % SDS, 0.002 % Bromophenol blue, 5 % beta-mercaptoethanol).
Handling Advice	Minimize freeze-thaw cycles.
Storage	-80 °C
Storage Comment	Store at -80 °C. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20 °C.
Expiry Date	6 months

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