產品詳情

  • 產品名稱：KRAS293CellLysate

  • 產品型號：
  • 產品廠商：Abbiotec
  • 產品文檔：
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KRAS293CellLysate
詳情介紹：
Characteristics Antigen standard for v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (True ORF cDNA clone RC201958, OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Components This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer.
Alternative Name KRAS
Background Synonyms: Kirsten rat sarcoma-2 viral (v-Ki-ras2) oncogene homolog, v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog, transforming protein p21, c-Kirsten-ras protein, K-ras p21 protein, oncogene KRAS2, KRAS, C-K-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS, NS3, RASK2
NCBI Accession NP_004976
Application Notes WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 μg lysate per lane.
Restrictions For Research Use only
Format Liquid
Buffer Each lysate vial contains 0.1 mg lysate in 0.1 mL (1 mg/mL) of RIPA Buffer (50 mM Tris-HCl pH 7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1 % NP40). The loading buffer vial contains 0.5 mL 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10 % glycerol, 4 % SDS, 0.002 % Bromophenol blue, 5 % beta-mercaptoethanol).
Handling Advice Minimize freeze-thaw cycles.
Storage -80 °C
Storage Comment Store at -80 °C. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20 °C.
Expiry Date 6 months