產(chǎn)品詳情

  • 產(chǎn)品名稱：DDX39293CellLysate

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  • 產(chǎn)品廠商：Abbiotec
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DDX39293CellLysate
詳情介紹：
Characteristics Antigen standard for DEAD (Asp-Glu-Ala-Asp) box polypeptide 39 (DDX39) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Components This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer.
Alternative Name DDX39
Background Synonyms: nuclear RNA helicase, DECD variant of DEAD box family, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 39, UAP56-related helicase, 49 kDa, DEAD (Asp-Glu-Ala-Asp) box polypeptide 39 transcript, DEAD box protein 39, nuclear RNA helicase URH49, DDX39, BAT1, BAT1L, DDXL, MGC18203, MGC8417, URH49
NCBI Accession NP_005795
Research Area Chromatin and Nuclear Signaling, Transcription Factors, DNA/RNA, Cancer
Application Notes WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 μg lysate per lane.
Restrictions For Research Use only
Format Liquid
Buffer Each lysate vial contains 0.1 mg lysate in 0.1 mL (1 mg/mL) of RIPA Buffer (50 mM Tris-HCl pH 7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1 % NP40). The loading buffer vial contains 0.5 mL 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10 % glycerol, 4 % SDS, 0.002 % Bromophenol blue, 5 % beta-mercaptoethanol).
Handling Advice Minimize freeze-thaw cycles.
Storage -80 °C
Storage Comment Store at -80 °C. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20 °C.
Expiry Date 6 months