產(chǎn)品詳情

  • 產(chǎn)品名稱：Mouseanti-HumanIgMAntibody

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  • 產(chǎn)品廠商：NSJBio
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Mouseanti-HumanIgMAntibody
詳情介紹：
Immunogen Recombinant heavy chain of human IgM (IM260 IM373 & ICO-30) was used as the immunogen for the Anti-IgM antibody cocktail.
Clone IM260-IM373-ICO-30
Isotype IgG1, IgG2b, kappa
Purification Protein G affinity chromatography
Background Recognizes a protein of 75?kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This mAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Research Area Secondary Antibodies
Application Notes Optimal dilution of the Anti-IgM antibody cocktail should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10?mM Citrate buffer,?pH 6.0, for 10-20?min followed by cooling at RT for 20?min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30?min.\. Flow Cytometry: 0.5-1?μg/million cells in 0.1ml,Immunofluorescence: 0.5-1?μg/mL,Western blot: 0.5-1?μg/mL,Immunohistochemistry (FFPE): 0.5-1?μg/mL for 30?min at RT (1),Prediluted format : incubate for 30?min at RT (2)
Restrictions For Research Use only
Buffer 1?mg/mL in 1X PBS, BSA free, sodium azide free
Preservative Azide free
Storage 4 °C,-20 °C
Storage Comment Store the Anti-IgM antibody c°Cktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).